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| Id: | 19627
| | Author: | Sakai, Tetsuo; Matsuo, Eiichi; Wakizaka, Akira. | | Title: | Complete DNA sequence analysis for 16s ribosomal RNA gene of the leproma-derived, cultivable and nerve-invading mycobacterium HI-75.
| | Source: | Int. J. Lepr;67(1):52-59, Mar., 1999. tab.
| | Abstract: | The complete 1493 nucleotide sequence of the 16SrRNA gene of the leproma-derived and cultivable mycobacterium HI-75 strain was analyzed to elucidate the taxonomic characteristics by direct sequencing of the polymerase chain reaction (PCR) products. The results revealed that the sequence of mycobacterium HI-75 was mostly similar to that of Mycobacterium scrofulaceum with 5 bases differences in the sequenced 1493 bases (0.35%) of the 16SrRNA gene. M. leprae differed from the strain with 47 bases (3.3%). Sasaki and Hamit reported the nerve-invasive activity of the inoculated mycobacterium HI-75 in nude mice or the 131I-treated immunocompromised Swiss mice. The results indicate that mycobacterium HI-75 could be a mutant of M. scrofulaceum possessing the ability to invade the peripheral nerve in addition to developing leproma-like lesions. (AU)^ien.
| | Descriptors: | DNA/genet
RNA Ribossômico/genet
RNA Ribossômico/imunol
Mycobacterium leprae/genet
| | Electronic Medium: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n1/v67n1a08.pdf / en
| | Location: | BR191.1 |
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